All operations are performed on ice. The separated cytoplasmic protein and nuclear protein were subjected to Western blot analysis. Grouper spleen cells were transfected with pEGFP-C1, pEGFP-LC3, ...

The mRNA expressions of let-7f, autophagy-associated genes (mTORC1, Beclin-1, ATG12, ATG5, and LC3), Runx2, and CTSK were examined. In vitro, the let-7f overexpression/silencing vector was constructed ...

Methods Mitochondria were evaluated in peripheral blood lymphocytes (PBL) of 38 SLE patients and 21 healthy controls and mouse models by flow cytometry, microscopy and western blot. MRL/lpr mice were ...

Does this suggest lysosome dysfunction in TRGL-Veh mice? (5) In Figure 5A, the phosphorylation of ATG14 (S29) shows minimal differences in Western blotting, which appears inconsistent with the ...

The best Western shirts also have fancy details like pearl snap buttons (that don’t come off as easily as regular buttons), and handy chest pockets that, while once used for cowboy essentials ...

The Western genre is one of the oldest and cherished film genres to ever enter the landscape of cinema and successfully transitioned into the Talkies, only growing immensely popular among movie goers.

Both television and cinematic content of the ever-evolving Western genre have found their place in the digital library of Netflix, which houses some of the most compelling TV shows in the genre. This ...

肺泡上皮细胞损伤、肺成纤维细胞的增殖与激活，是诱导肺纤维化的关键因素 。之前的研究发现，表面活性剂蛋白相关基因和端粒相关基因的变异，可能会导致肺泡上皮细胞受损，进而引发 IPF 。肺损伤后，成纤维细胞被激活并转化为肌成纤维细胞，促使包括胶原蛋白、α - 平滑肌肌动蛋白（α - SMA）和波形蛋白（Vim）等纤维化标志物的产生，最终形成瘢痕，导致 IPF ...