组织块连续贴壁传代培养（continuous adherence subculture of tissue block ，CASTB）在一些 珍贵材料 ， 对消化敏感 ， 极性较高 ， 分化程度较高 的组织（例如人的体组织材料，分化程度较高或代极性的组织附睾，睾丸，心脏，脑等）等适合用组织块连续贴壁传代培养获得 ...

2023年10月1日 · In the present study, we explored a newly improved method of cell culture. Firstly, tissue block was continuously subcultured for several passages to achieve cells; as the control, meanwhile, the cells from tissue block at passage …

2023年8月26日 · Methods: Dermal tissue blocks were obtained from BALB/c neonatal mice and randomly divided into experimental group and control group. The experimental group received the newly improved culture method, namely, continuous adherence subculture of tissue block (CASTB) method; while the traditional subculture method was applied in the control group.

Tissue block culture method refers to a cell-cultured approach that proper-size tissue blocks, after tightly attaching to the wall, grow in the serum-coated culture ask to generate primary cells (Mohd

方法 ·取健康牙龈组织，利用组织块法体外培养hGFs，免疫荧光染色验证组织来源；连续传代培养，计算累积细胞群体倍增指数（CPD）并绘制曲线；取CPD值为10.82、20.65、29.52、42.22、60.79、70.03的hGFs，CCK-8试剂盒检测细胞增殖能力改变，real-time PCR检测衰老相关基因p16INK4a、p21Cip1的表达变化。 结果...

2022年12月1日 · In this study, "the selective secondary tissue attachment method” was used for isolation and purification of bovine MECs (bMECs). It was based on the tissue block method and employs bovine mammary gland tissue. This method is centered on the fact that different cell types isolated from mammary tissues exhibit different migration rates.

2023年1月13日 · Tissue block culture method refers to a cell-cultured approach that proper-size tissue blocks, after tightly attaching to the wall, grow in the serum-coated culture flask to generate primary cells (Mohd et al. 2017; Lehmann et al. 2018; Na et al. 2021; Nichols et al. 2021; Tam et al. 2022). This method is simple and stable.

2016年3月29日 · Methods: Skeletal muscle satellite cells were obtained by tissue block culture method in combination with pre-plating techniques, and the purity of these cells was detected by both immunocytochemistry and fluorescence activated cell sorter (FACS) with Pax7 as …

Tissue block culture method: The obtained tumor tissue was removed from the fat\connective tissue and necrotic part, washed with Hanks' solution 3 times in a flat dish, minced the tissue, cut into 1~2mm 3 small pieces, inoculated in a culture flask (or dish) coated with rat tail collagen in advance, and cultured in an ordinary incubator at 37 ...

2023年1月10日 · The experimental group received the newly improved culture method, namely, continuous adherence subculture of tissue block (CASTB) method; while the traditional subculture method was...