2016年3月7日 · Here, we present an approach to produce cleavable antibody-DNA conjugates, employing the fast kinetics of the inverse electron-demand Diels-Alder reaction between tetrazine and trans...

2020年1月29日 · In this study, we combined a non-site-directed antibody conjugation technique using copper-free click chemistry with ion-exchange chromatography to obtain purified single and double...

2021年7月2日 · Herein, we provide optimized protocols for conjugating purified antibodies to DNA oligonucleotides, validating the conjugation by CODEX staining and executing the CODEX multicycle imaging...

Antibody–oligonucleotide conjugates (AOCs) combine the high precision of siRNA and ASOs with the deliverability of antibodies, thus synergizing the advantages of both technologies. The field of AOCs started initially as a means to develop powerful diagnostic tools but has evolved more recently as a targeted therapeutic approach for many ...

2021年9月23日 · Here, we report a new platform for the preparation of DNA-antibody bioconjugates with a simple benzoylacrylic acid pentafluorophenyl ester reagent. Benzoylacrylic-labelled oligonucleotides prepared with this reagent can be site-specifically conjugated to a range of proteins and antibodies through accessible cysteine residues.

2019年8月22日 · Here we present a universal, site-selective conjugation method using a small photo-cross-linkable protein G adaptor that allows labeling of antibodies of different host species with a controlled number of short oligonucleotides (ODNs).

2019年1月4日 · Using a strain-promoted azide-alkyne cycloaddition (SPAAC), we synthesized covalent DNA-antibody conjugates, optimized the reaction conditions, and developed an efficient protocol for the purification of conjugates, with which all …

In this review, we will first briefly introduce the methods for constructing and purifying DNA-antibody conjugates. Then, we will focus on the application of DNA-antibodies in protein detection and quantification, primarily based on immuno-PCR.

2012年2月15日 · Here we describe the synthesis of site-specific antibody-oligonucleotide conjugates using genetically encoded unnatural amino acids (UAAs) with unique chemical reactivity.

2024年11月22日 · A DNA-antibody conjugate combines the unique functions of both antibodies and DNA in a single molecule, allowing for a wide range of applications. These applications range from ultrasensitive protein measurements based on PCR to the spatial detection of biomolecules.